A number of different methods, technologies, and protocols are available for performing the actual cloning reaction. To choose the one that best meets your needs, you will need to analyze the current parameters of your experiment and plan ahead for any downstream procedures. This section discusses several highly efficient cloning technologies:
Restriction Enzyme Digestion & Ligation Cloning: is an industry standard for molecular biologistsTOPO Cloning: provides simple, convenient reactions typically in less than 5 minGateway Cloning Technology offers the greatest flexibility in vector choices and downstream applicationsSeamless Cloning and Assembly: Tools optimized to clone up to 4 DNA fragments simultaneously into virtually any linearized E. coli vector in a 30 minute room temperature reactionType IIs Assembly: Clone up to 8 fragments simultaneously and without scars, and avoid rearrangements when cloning homologous or repetitive sequencesGeneArt Gene Synthesis and Strings DNA Fragments: 100% accuracy and optimization of genes to maximize expression The table below can help you navigate your way through these technologies.
View our Cloning Technologies Guide